Device

Part:BBa_K4365021:Design

Designed by: Giorgio Gilioli   Group: iGEM22_TU_Dresden   (2022-09-30)


SP-SUMO-turboRFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1001
    Illegal PstI site found at 1036
    Illegal PstI site found at 1335
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1001
    Illegal PstI site found at 1036
    Illegal PstI site found at 1335
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2168
    Illegal BamHI site found at 1007
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1001
    Illegal PstI site found at 1036
    Illegal PstI site found at 1335
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1001
    Illegal PstI site found at 1036
    Illegal PstI site found at 1335
    Illegal NgoMIV site found at 172
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

...

Source

Construct was ordered from IDT as gBlock, cloned into the p426 plasmid and fused to the N-terminus of the turboRFP fluorescent protein.

References